Protocols - MS Analysis of Purified Proteins (Samples with Low Complexity)
Whether you want to confirm the identity of your purified protein or to identify a novel protein modification, MS characterization can provide unambiguous evidence. To analyze highly purified proteins we recommend to follow the in-solution protocol. If abundant contaminants such as serum albumin or detergents are present, it might be advantageous to separate the proteins by SDS-PAGE, cut out the protein band and perform an in-gel digest.
At the moment our facility is not equipped to perform top-down proteomics of native proteins with molecular weights larger than 30 kDa.
In solution digest
Dependent peptide search