Imaging Center

Epifluorescence Microscope: Leica – THUNDER Imager 3D Live Cell (2021)

Leica's THUNDER Imager 3D Live Cell is an inverted epifluorescence microscope for multi-colour imaging of live and fixed cells. It is equipped with an incubator including temperature- and CO2-control modules. Based on the use of computational clearing this microscope can also be used to resolve details in thick samples in real time.

Microscope details
General applicationsMulti-colour imaging of fixed and live cells, especially used for yeast cells
Extra features

Computational clearing for imaging thick samples
(instant / small volume / large volume computational clearing), DIC

Microscope body

DMI8 inverted

fully motorised

Illumination
High speed LED8 with high speed emission filter wheel
(8 LEDs: 395, 438, 475, 511, 555, 575, 635, 730 nm
Filter cubesLED8 emission filter wheel (460/80; 535/70; 590/50; 642/80);
Filter cube CYR71010 (excitation: 436/28, 506/21, 578/24, 730/40;
dichroic mirror: 459, 523, 598, 763; emission: 473/22, 539/24, 641/78, 810/80);
Filter cube DFT51010 (excitation: 391/32, 479/33, 554/24, 638/31;
dichroic mirror: 415, 500, 572, 660; emission: 435/30, 519/25, 594/32, 695/58)
DetectionLeica DFC9000 GTC (4.2 MP monochrome sCMOS camera with 82% max QE;
6.5 µm pixel size)
ObjectivesN PLAN 5x/0.12 PH0
HC PL FLUOTAR 10x/0.32 PH1
HC PL FLUOTAR L 40x/0.60 CORR PH 2
HC PL APO 40x/1.30 Oil
HC PL APO 100x/1.44 Oil CORR CS1150
Focus systemHardware autofocus Leica AFC including closed loop focus
IncubatorIncubator i8 with temperature- and CO2-control
SoftwareLAS X (Live Data Mode, Trigger to peripherals, 3D Analysis, 3D Visualization Advanced)
Acquisition year2021 (DFG, INST 248/336-1)
Location

Department of Cell Biology
Building 13, Room 13-468

ContactProf. Dr. Johannes Herrmann,
0631 205 2406

 

 

 

 

 

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