Imaging Center

Confocal Laser Scanning Microscope

Zeiss - LSM 880 (2017)

The LSM 880 is an inverted confocal laser scanning microscope. For live cell imaging the microscope is equipped with a temperature-controlled incubator and a CO2 module.

Image of Zeiss LSM880 microscope
LSM 880 Setup
Zeiss LSM880 microscope Image of roots
Vacuoles in cortex cells in the root tip of Arabidopsis thaliana were stained with the fluorescent pH indicator BCECF and with the vacuole reporter spRFP-AFVY (courtesy of David Scheuring).

Microscope details

General
applications		High resolution multi-colour imaging of fixed cells
Fast high resolution multi-colour imaging of living cells
Extra
features

Time lapse
Multiwell FRAP
Located in S2 room

Microscope
body		Inverted
Axio Observer
IlluminationLaser (405, 488, 514, 543, 594, 633 nm)
LED light source Colibri
Filter cubes 
DetectionQuasar detector (spectral detector, GaAsP PMT) for high sensitivity
Transmitted light PMT
Objectives

Plan-Apochromat 5x/0.16
Plan-Apochromat 20x/0.8
C Apo 40x/1.2 W
Long Distance (LD) LCI Plan-Apochromat 40x/1.2 W
Pln Apo 63x1.4 oil

Focus system 
IncubatorYes, temperature-control, CO2-module
SoftwareImaris for data analysis available
Acquisition
year		2017 (funded by DFG: INST 248/254-1)
LocationDepartment of Microbiology
Building 23, Room 161
ContactSusanne Zehner,
0631 205 2503

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