Imaging Center

Epifluorescence Microscope

Nicon Eclipse Ti2-E (2020)

The Nicon Eclipse Ti2-E is an inverted epifluorescence microscope which is equipped with a control unit for CO2 and temperature. Together with its reliable Perfect Focus System (PFS4) this makes it perfect for long-term time lapse experiments with mammalian cells. The LED light source Spectra III has eight lines covering a large excitation range. With the present filter sets 4 dyes can be imaged simultaneously (e.g. Dapi, A488, A568, A647).

Image of Nikon Eclipse Ti2-E microscope
Nicon Eclipse Ti2-E setup
Picture taken by Nicon Microscope: Mouse embryonic fibroblasts labeled in red with phalloidin to visualize actin and in green with paxillin-specific antibodies to reveal focal adhesions. Nuclei are depicted in blue (courtesy of Fabian Lukas)
Mouse embryonic fibroblasts labeled in red with phalloidin to visualize actin and in green with paxillin-specific antibodies to reveal focal adhesions. Nuclei are depicted in blue (courtesy of Fabian Lukas)

Microscope details

General
applications

4-colour imaging of fixed and live mammalian cells based on dyes such as Dapi (405) and Alexa (488, 568, 647)
Long-term multi-position time lapse with stable focus

Extra
features

Z-stack
Phase contrast objectives
Additional 1.5x magnification lense

Microscope
body		Ti2-E inverted, motorized
IlluminationLumencor Spectra III 8-position LED light source with 8 fixed excitation filters (395/25; 438/29; 475/28; 511/16; 555/28; 575/25, 635/22, 748/12)
Filter cubes

DAPI/A350; FITC/A488; TRITC/A546; Cy5/A647
Alternatively single bandpass filters to prevent bleed-through or multi bandpass quad band filter for increased speed

DetectionMono DS-Qi2, monochrome digital camera with "FX" CMOS sensor
Objectives

20x (long distance objective), 60x (oil)

Focus systemPerfect Focus System PFS4 (offset lense)
IncubatorYes, CO2 and temperature control
SoftwareNIS Elements AR with various plug-ins
Acquisition
year		2020
LocationDepartment of Nanophysiology
Building 13, Room 155.3
ContactTanja Maritzen,
0631 205 4908

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