Imaging Center

Epifluorescence Microscope

Zeiss - High Speed Cell Observer with ApoTome (Axio Observer Z.1; 2009)

The High Speed Cell Observer microscope is an inverted epifluorescence microscope with ApoTome module for structured illumination to obtain optical sections. The microscope is equipped for live cell imaging with a temperature-controlled incubator and a CO2 module. It can be used for routine imaging of fixed and living cells.

Image of Zeiss High Speed Cellobserver microscope
High Speed Cell Observer setup
Zeiss High Speed Cellobserver microscope Image of HeLa cells
Overlay image of permeabilized HeLa cells stained with antibodies against myc-APP (green) and HA-Neuroligin1-AB (courtesy of Simone Eggert)

Microscope details

General
applications

Multi-colour imaging of fixed and live mammalian cells based on dyes such as DAPI (405 nm) and Alexa (488, 568, 594, 647 nm)
Interaction studies

Extra
features		ApoTome, time lapse imaging, 3D display, colocalization, Z-stack, tiles + positions (widefield) => creation of images that are larger than the actual image section
Microscope
body		Inverted
Illumination

LED light source Colibri
HXP (fluorescence)
Halogen (transmitted light)

Filter cubes

GFP (green) = set38 (BP 470/40, FT 495, BP 525/50);
PI (red): set00 (BP 530-585, FT 600, LP 615)

DetectionAxiocam 702 Novo
Objectives

20x, 40x, 63x (oil), 63x (glycerol), 100x (oil)

Focus systemDefinite Focus autofocus system
IncubatorYes, temperature-control, CO2-module
SoftwareZen blue (Zeiss)
Acquisition
year		2009
Location

Human Biology and Human Genetics
Building 13 Room 103

Contact

Stefan Kins,
0631 205 2106

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