Imaging Center

Confocal Laser Scanning Microscope

Leica - TCS SP5 II (2011)

The TCS SP5 II is a confocal laser scanning microscope for high resolution multi-colour imaging.

Image of Leica TCS SP5II Bioprocess Engineering microscope
Leica TCS SP5II setup
Leica TCS SP5II microscope Image of cyanobacteria
Image of adherently growing cyanobacteria (red; courtesy of Judith Stiefelmaier and Dorina Strieth)

Microscope details

General
applications

Multi-color imaging of eukaryotic and procaryotic cells with fluorophores

FRAP on biofilms

Extra
features		FRAP, FRET, motorized stage
Microscope
body

Upright
Motorized

IlluminationLaser (405, 458, 476, 488, 514, 561, 633 nm)
Filter cubes 
Detection

2x HyD, 3x PMT

Objectives

10x0.3, 25x0.95W, 50x0.9, 63x1.2W, 63x1.4O, 63x0.9W, 100x0.95

Focus system 
IncubatorTemperature control
SoftwareLeica LAS AF
Acquisition
year		2011 (funded by DFG)
LocationBioprocess Engineering
Building 56, Room 311
ContactJudith Stiefelmaier,
0631 205 5576

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