Imaging Center

2-Photon Microscope

Bruker - Ultima Investigator (2016/2017)

The Ultima Investigator is a 2-photon microscope and thus enables a deeper tissue penetration than regular confocal microscopes. Therefore it is well suited for the imaging of living tissue in an intact organ such as fluorescently labeled neurons in the brain of an anesthesized mouse.

Image of Bruker Coherent Ultima Investigator microscope
Bruker Ultima Investigator setup
Bruker coherent ultima investigator microscope Image of labeled neurons in intact brain
Labeled neurons in intact brain observed by two-photon microscopy (courtesy of Jan Hirtz)

Microscope details

General
applications

Microscopy of cells in an intact, living organism with enhanced tissue penetration

Extra
features

Tilting objective
Acoustic stimulation equipment
Aound attenuation chamber
Patch-clamp setup

Microscope
body		Bruker Ultima Investigator
Illumination

Coherent Chameleon Vision II NIR-Laser green diode transmitted light illumination for translucent samples 

Filter cubes 
Detection

Two GaAsP PMTs
Photometrics Prime sCMOS camera

Objectives4x, 10x, 16x, 20x, 40x
Focus systemMotor-based
IncubatorNo incubator
SoftwarePrairie View
Acquisition
year		2016/17 (funded by DFG INST 248/222-1 FUGG)
LocationDepartment of Animal Physiology
Building 13, Room 547
ContactJan Hirtz,
0631 205 4669

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