Imaging Center

Confocal Laser Scanning Microscope

Zeiss/3i Marianas (2008)

The Marianas microscopy platform is an inverted confocal laser scanning microscope for high resolution multi-colour imaging. It is equipped with a heated block, but not with a CO2 chamber.

Image of Zeiss+3i marianas microscope
Zeiss+3i Marianas setup
Zeiss 3i Marianas microscope Image of human cell during anaphase
Image showing a human cell during anaphase with ultrafine anaphase bridge that arises due to defective DNA repair or replication. The chromosomes (green) are labeled with histone H2B-GFP, the ultrafine bridges are stained with an antibody against the Bloom helicase BLM (courtesy of Divya Reddy)

Microscope details

General
applications

Microscopy of fixed mammalian cells based on dyes such as DAPI (405) and Alexa (488, 594, 647)

Extra
features		Transmitted light T-PMT
Microscope
body		 
IlluminationLaser (488, 560, 640 nm; UV)
Fluorescence illumination
Filter cubes528 nm, 617 nm, 460 nm, 705 nm
Detection

Cool Snap HQ2
Cool Snap Ez

Objectives

10x, 20x, 40x, 63x (oil), 100x (oil)

Focus system 
IncubatorHeated block
SoftwareSlidebook
Acquisition
year		2008
LocationDepartment of Molecular Genetics
Building 24, Room 135C
ContactZuzana Storchová,
0631 205 3250

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